@article {677016,
	title = {Fixed single-cell transcriptomic characterization of human radial glial diversity.},
	journal = {Nat Methods},
	volume = {13},
	number = {1},
	year = {2016},
	month = {2016 Jan},
	pages = {87-93},
	abstract = {The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1\% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.},
	issn = {1548-7105},
	doi = {10.1038/nmeth.3629},
	author = {Thomsen, Elliot R and Mich, John K and Yao, Zizhen and Hodge, Rebecca D and Doyle, Adele M and Jang, Sumin and Shehata, Soraya I and Nelson, Angelique M and Shapovalova, Nadiya V and Levi, Boaz P and Ramanathan, Sharad}
}
